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IL-5 induced Gαi1/3 association with activated IL-5 receptor and Gab1. WT and Gαi1/3 DKO MEFs were treated with IL-5 (25 ng/mL) for 5 min, the association between <t>IL-5Rα,</t> Gab1, and Gαi1/3 was tested by co-ip assay (A) . EoL-1 cells were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 5 min, the association between IL-5Rα, Gab1, and Gαi1/3 was tested by co-ip (B) , and processed for confocal microscopy to detect the presence of Gαi1, Gαi3, and IL-5Rα (C) . Bar = 1 μm.
Il 5rα, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/il 5rα/product/Bioss
Average 91 stars, based on 1 article reviews
il 5rα - by Bioz Stars, 2026-03
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cd125 biotinstreptavidin bv421  (Miltenyi Biotec)
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IL-5 induced Gαi1/3 association with activated IL-5 receptor and Gab1. WT and Gαi1/3 DKO MEFs were treated with IL-5 (25 ng/mL) for 5 min, the association between <t>IL-5Rα,</t> Gab1, and Gαi1/3 was tested by co-ip assay (A) . EoL-1 cells were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 5 min, the association between IL-5Rα, Gab1, and Gαi1/3 was tested by co-ip (B) , and processed for confocal microscopy to detect the presence of Gαi1, Gαi3, and IL-5Rα (C) . Bar = 1 μm.
Cd125 Biotinstreptavidin Bv421, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd125 biotinstreptavidin bv421/product/Miltenyi Biotec
Average 93 stars, based on 1 article reviews
cd125 biotinstreptavidin bv421 - by Bioz Stars, 2026-03
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p stat5  (Bioss)
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Bioss p stat5
Gαi1/3 knockdown inhibits <t>STAT5</t> activation, differentiation and growth by IL-5 in EoL-1 cells. Stable EoL-1 cells, expressing the scramble control shRNA (“C-shRNA”), Gαi1 shRNA and Gαi3 shRNA, were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 24 hours, and were tested by Western blotting of listed proteins (A, E) . Relative expression of listed genes (24 hours after GM-CSF and IL-5 treatment) was shown (B, C) ; In each experiment, n=3 (three replicated wells). Cells were further cultured for the times indicated, cell proliferation were tested (F, G) , with cell viability tested by CCK-8 assay (D) . Bar = 100 μm. Blotting quantification was performed from five replicate blot data, data of all repeated experiments were pulled together to calculate mean ± SD. *P <0.05 (B, E, G) . *P <0.05 vs. “Control” treatment in “C-shRNA” EoL-1 cells (C) . # P <0.05 vs. GM-CSF and IL-5 treatment in “C-shRNA” EoL-1 cells (C) . *P <0.05 vs. “Control” treatment in “Gαi1/3-shRNA” EoL-1 cells (D) . #P <0.05 vs. GM-CSF and IL-5 treatment in “Gαi1/3-shRNA” EoL-1 cells (D) .
P Stat5, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p stat5/product/Bioss
Average 91 stars, based on 1 article reviews
p stat5 - by Bioz Stars, 2026-03
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rea343  (Miltenyi Biotec)
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Miltenyi Biotec rea343
Gαi1/3 knockdown inhibits <t>STAT5</t> activation, differentiation and growth by IL-5 in EoL-1 cells. Stable EoL-1 cells, expressing the scramble control shRNA (“C-shRNA”), Gαi1 shRNA and Gαi3 shRNA, were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 24 hours, and were tested by Western blotting of listed proteins (A, E) . Relative expression of listed genes (24 hours after GM-CSF and IL-5 treatment) was shown (B, C) ; In each experiment, n=3 (three replicated wells). Cells were further cultured for the times indicated, cell proliferation were tested (F, G) , with cell viability tested by CCK-8 assay (D) . Bar = 100 μm. Blotting quantification was performed from five replicate blot data, data of all repeated experiments were pulled together to calculate mean ± SD. *P <0.05 (B, E, G) . *P <0.05 vs. “Control” treatment in “C-shRNA” EoL-1 cells (C) . # P <0.05 vs. GM-CSF and IL-5 treatment in “C-shRNA” EoL-1 cells (C) . *P <0.05 vs. “Control” treatment in “Gαi1/3-shRNA” EoL-1 cells (D) . #P <0.05 vs. GM-CSF and IL-5 treatment in “Gαi1/3-shRNA” EoL-1 cells (D) .
Rea343, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rea343/product/Miltenyi Biotec
Average 93 stars, based on 1 article reviews
rea343 - by Bioz Stars, 2026-03
93/100 stars
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cd125 (il-5rα) antibody, anti-mouse, pe, reafinity  (Miltenyi Biotec)
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Miltenyi Biotec cd125 (il-5rα) antibody, anti-mouse, pe, reafinity
Gαi1/3 knockdown inhibits <t>STAT5</t> activation, differentiation and growth by IL-5 in EoL-1 cells. Stable EoL-1 cells, expressing the scramble control shRNA (“C-shRNA”), Gαi1 shRNA and Gαi3 shRNA, were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 24 hours, and were tested by Western blotting of listed proteins (A, E) . Relative expression of listed genes (24 hours after GM-CSF and IL-5 treatment) was shown (B, C) ; In each experiment, n=3 (three replicated wells). Cells were further cultured for the times indicated, cell proliferation were tested (F, G) , with cell viability tested by CCK-8 assay (D) . Bar = 100 μm. Blotting quantification was performed from five replicate blot data, data of all repeated experiments were pulled together to calculate mean ± SD. *P <0.05 (B, E, G) . *P <0.05 vs. “Control” treatment in “C-shRNA” EoL-1 cells (C) . # P <0.05 vs. GM-CSF and IL-5 treatment in “C-shRNA” EoL-1 cells (C) . *P <0.05 vs. “Control” treatment in “Gαi1/3-shRNA” EoL-1 cells (D) . #P <0.05 vs. GM-CSF and IL-5 treatment in “Gαi1/3-shRNA” EoL-1 cells (D) .
Cd125 (Il 5rα) Antibody, Anti Mouse, Pe, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd125 (il-5rα) antibody, anti-mouse, pe, reafinity/product/Miltenyi Biotec
Average 93 stars, based on 1 article reviews
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cd125 (il5ra) bd 555902 antibody  (Becton Dickinson)
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Becton Dickinson cd125 (il5ra) bd 555902 antibody
( A ) <t>CD125</t> but not CD69 ( B ) or CD193 ( C ) was increased in circulating eosinophils from ER patients versus healthy donors. * p < 0.05. Each dot represents a unique donor. N = 7 ER; N = 10 HD.
Cd125 (Il5ra) Bd 555902 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd125 (il5ra) bd 555902 antibody/product/Becton Dickinson
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cd125 biotin  (Miltenyi Biotec)
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( A ) <t>CD125</t> but not CD69 ( B ) or CD193 ( C ) was increased in circulating eosinophils from ER patients versus healthy donors. * p < 0.05. Each dot represents a unique donor. N = 7 ER; N = 10 HD.
Cd125 Biotin, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd125 biotin/product/Miltenyi Biotec
Average 93 stars, based on 1 article reviews
cd125 biotin - by Bioz Stars, 2026-03
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human anti cd125 il5ra biotin validation  (Miltenyi Biotec)
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( A ) <t>CD125</t> but not CD69 ( B ) or CD193 ( C ) was increased in circulating eosinophils from ER patients versus healthy donors. * p < 0.05. Each dot represents a unique donor. N = 7 ER; N = 10 HD.
Human Anti Cd125 Il5ra Biotin Validation, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human anti cd125 il5ra biotin validation/product/Miltenyi Biotec
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human anti cd125 il5ra biotin validation - by Bioz Stars, 2026-03
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anti cd125 il 5rα apc  (Miltenyi Biotec)
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Miltenyi Biotec anti cd125 il 5rα apc
( A ) <t>CD125</t> but not CD69 ( B ) or CD193 ( C ) was increased in circulating eosinophils from ER patients versus healthy donors. * p < 0.05. Each dot represents a unique donor. N = 7 ER; N = 10 HD.
Anti Cd125 Il 5rα Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd125 il 5rα apc/product/Miltenyi Biotec
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Image Search Results


IL-5 induced Gαi1/3 association with activated IL-5 receptor and Gab1. WT and Gαi1/3 DKO MEFs were treated with IL-5 (25 ng/mL) for 5 min, the association between IL-5Rα, Gab1, and Gαi1/3 was tested by co-ip assay (A) . EoL-1 cells were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 5 min, the association between IL-5Rα, Gab1, and Gαi1/3 was tested by co-ip (B) , and processed for confocal microscopy to detect the presence of Gαi1, Gαi3, and IL-5Rα (C) . Bar = 1 μm.

Journal: Frontiers in Immunology

Article Title: Gαi1/3 signaling mediates IL-5-induced eosinophil activation and type 2 inflammation in eosinophilic chronic rhinosinusitis

doi: 10.3389/fimmu.2024.1460104

Figure Lengend Snippet: IL-5 induced Gαi1/3 association with activated IL-5 receptor and Gab1. WT and Gαi1/3 DKO MEFs were treated with IL-5 (25 ng/mL) for 5 min, the association between IL-5Rα, Gab1, and Gαi1/3 was tested by co-ip assay (A) . EoL-1 cells were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 5 min, the association between IL-5Rα, Gab1, and Gαi1/3 was tested by co-ip (B) , and processed for confocal microscopy to detect the presence of Gαi1, Gαi3, and IL-5Rα (C) . Bar = 1 μm.

Article Snippet: Antibodies against Gαi1 (sc-13533), Gαi2 (sc-13534), Gαi3 (sc-365422), Gab1 (sc-133191), Akt (sc-81434), Erk1/2 (sc-514302), S6K (sc-8418), p-Gab1 (AP0256), p-Akt s473 (sc-101629), p-Erk1/2 (sc-136521), p-S6K (sc-8416), STAT5 (sc-74442), p-STAT5 (AP-0887), IL-5Rα (bs-2601R-100ul), IgE (ab75673), and tryptase (ab2378) were purchased from Bioss (Woburn, MA, USA), ABcolnal (Wuhan, China), Abcam (Cambridge, MA, USA), and Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Co-Immunoprecipitation Assay, Confocal Microscopy

Gαi1/3 knockdown inhibits STAT5 activation, differentiation and growth by IL-5 in EoL-1 cells. Stable EoL-1 cells, expressing the scramble control shRNA (“C-shRNA”), Gαi1 shRNA and Gαi3 shRNA, were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 24 hours, and were tested by Western blotting of listed proteins (A, E) . Relative expression of listed genes (24 hours after GM-CSF and IL-5 treatment) was shown (B, C) ; In each experiment, n=3 (three replicated wells). Cells were further cultured for the times indicated, cell proliferation were tested (F, G) , with cell viability tested by CCK-8 assay (D) . Bar = 100 μm. Blotting quantification was performed from five replicate blot data, data of all repeated experiments were pulled together to calculate mean ± SD. *P <0.05 (B, E, G) . *P <0.05 vs. “Control” treatment in “C-shRNA” EoL-1 cells (C) . # P <0.05 vs. GM-CSF and IL-5 treatment in “C-shRNA” EoL-1 cells (C) . *P <0.05 vs. “Control” treatment in “Gαi1/3-shRNA” EoL-1 cells (D) . #P <0.05 vs. GM-CSF and IL-5 treatment in “Gαi1/3-shRNA” EoL-1 cells (D) .

Journal: Frontiers in Immunology

Article Title: Gαi1/3 signaling mediates IL-5-induced eosinophil activation and type 2 inflammation in eosinophilic chronic rhinosinusitis

doi: 10.3389/fimmu.2024.1460104

Figure Lengend Snippet: Gαi1/3 knockdown inhibits STAT5 activation, differentiation and growth by IL-5 in EoL-1 cells. Stable EoL-1 cells, expressing the scramble control shRNA (“C-shRNA”), Gαi1 shRNA and Gαi3 shRNA, were treated with GM-CSF (50 ng/mL) and IL-5 (50 ng/mL) for 24 hours, and were tested by Western blotting of listed proteins (A, E) . Relative expression of listed genes (24 hours after GM-CSF and IL-5 treatment) was shown (B, C) ; In each experiment, n=3 (three replicated wells). Cells were further cultured for the times indicated, cell proliferation were tested (F, G) , with cell viability tested by CCK-8 assay (D) . Bar = 100 μm. Blotting quantification was performed from five replicate blot data, data of all repeated experiments were pulled together to calculate mean ± SD. *P <0.05 (B, E, G) . *P <0.05 vs. “Control” treatment in “C-shRNA” EoL-1 cells (C) . # P <0.05 vs. GM-CSF and IL-5 treatment in “C-shRNA” EoL-1 cells (C) . *P <0.05 vs. “Control” treatment in “Gαi1/3-shRNA” EoL-1 cells (D) . #P <0.05 vs. GM-CSF and IL-5 treatment in “Gαi1/3-shRNA” EoL-1 cells (D) .

Article Snippet: Antibodies against Gαi1 (sc-13533), Gαi2 (sc-13534), Gαi3 (sc-365422), Gab1 (sc-133191), Akt (sc-81434), Erk1/2 (sc-514302), S6K (sc-8418), p-Gab1 (AP0256), p-Akt s473 (sc-101629), p-Erk1/2 (sc-136521), p-S6K (sc-8416), STAT5 (sc-74442), p-STAT5 (AP-0887), IL-5Rα (bs-2601R-100ul), IgE (ab75673), and tryptase (ab2378) were purchased from Bioss (Woburn, MA, USA), ABcolnal (Wuhan, China), Abcam (Cambridge, MA, USA), and Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Knockdown, Activation Assay, Expressing, Control, shRNA, Western Blot, Cell Culture, CCK-8 Assay

( A ) CD125 but not CD69 ( B ) or CD193 ( C ) was increased in circulating eosinophils from ER patients versus healthy donors. * p < 0.05. Each dot represents a unique donor. N = 7 ER; N = 10 HD.

Journal: Cells

Article Title: Granular Insights: Neutrophil Predominance and Elastase Release in Severe Asthma Exacerbations in a Pediatric Cohort

doi: 10.3390/cells13060533

Figure Lengend Snippet: ( A ) CD125 but not CD69 ( B ) or CD193 ( C ) was increased in circulating eosinophils from ER patients versus healthy donors. * p < 0.05. Each dot represents a unique donor. N = 7 ER; N = 10 HD.

Article Snippet: CD11b (BD 555386), L-selectin (BD 555542), and ICAM-1 (BD 555510) were measured in neutrophils, and CD125 (IL5Ra) (BD 555902), CD69 (BD 560738), and CD193 (BD 564189) were measured in eosinophils.

Techniques: